Method/procedure
for water biofilm Collection filtration (week 12)
Since last week I
never had the chance to share the methods/procedures for water bio-film
collection filtration, I’ll be delighted to do so this week.
500-1000
milliliter (mL) of first water was drawn. First draw water is the water
obtained immediately once the faucet is opened. Using two sterile swabs the
faucet, drain, or shower head was swabbed and placed in a 50-mL conical tube.
Using aseptic technique, the lower portion of the filter base was unwrapped,
which allowed us to insert the base of the rubber stopper in the neck of the
1-liter Erlenmeyer flask. Then, using flame-sterilized forceps, the membrane
filter on the filter base with the grid side up was placed aseptically. The
filtration apparatus ended by aseptically removing the glass receptacle from
the foil, and setting the lower surface on top of the membrane filter. The
filter funnel was clamped to the filter base allowing comfortable pouring of
250-500mL of the water sample or resuspended biofilm sample (50 mL) into the
receptacle. The sample was filtered by turning on the vacuum pump and turned
off when the sample was filtered entirely. After disassembling the top portion
of the filtration apparatus, a flame sterilized forceps were used to remove the
filter and placed grid-side up on the surface of the labeled PIA or PF agar
plate (for all samples). The PIA and PFA filtration plates were wrapped in
parafilm and incubated at 37°C.
Hi Ibrahim, your procedures and methods for water bio-film collection filtration are very well done! You were very thorough and precise with the units of measurements as well as the techniques you used during this procedure. I am excited to learn about everyone’s research during presentations. I am in the dark about most of the research projects but hopefully when the spring semester starts I will be able to actively keep up with everyone’s posts and research projects on blogspot. I am looking forward to learning about your research findings!
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