Extraction Point (week 6)
 |
Figure 1
|
As promised last week, the DNA extraction took place this week. All four of our Pseudomonas species (P. stutzeri, P. putida, P. aeruginosa, P. fluorescens) were extracted using different types of buffers (week five on my blog). After extracting DNA, our next step was to figure out how much DNA was extracted, which brings us to a NanoDrop spectrophotometer. The purpose of a NanoDrop spectrophotometer is to quantify the concentration of DNA present at a given solution. As shown below in figure 1, the concentration of DNA/Nucleic acid found in the extracted Pseudomonas fluorescence is found to be 2807.9 ng/µl. All the other Pseudomonas species had there concentrations of DNA figured out as well.
Hey Ibrahim, your nucleic acid values are really high so congratulations on doing a job well done! I know you mentioned the different kind of buffers you’d use but what kind of DNA extraction did you perform? Did you follow the same one throughout your bacteria or did you switch it up?
ReplyDeleteAs always, a wonderful job on explaining everything that we did, Ibrahim. I like how you bolded your specific bacteria species. It tells your audience which bacteria species is yours in a very subtle manner. I also like how you followed on what you’ve explained on your last blog. It shows coherence. Good job, all in all.
ReplyDelete