Pseudomonas Fluorescens (Week 3)
This week was based on studying the different species of Pseudomonas bacteria, specifically Pseudomonas Fluorescens. Pseudomonas for starters means “False unit,” a name given by early microbiologists inferring to ‘Germs.’ The specific name ‘Fluorescens’ refers to the microbe’s secretion of a soluble fluorescent pigment called ‘Pyoverdine,’ which is a type of siderophore (iron carriers usually secreted by bacteria’s) (Adams, 1985).
Here in the Biomedical Sciences Lab, we collected different kinds of ‘Pseudomonas’ that grew in various cultures. Our primary goal is to investigate the ‘Pseudomonas’ species that grow in different types of plastics. After gathering our Pseudomonas species, our next step was to culture them. We grew the different kinds of ‘Pseudomonas’’ in various temperatures (25°C, 37°C, and 42°C). My Pseudomonas’ species, Pseudomonas Fluorescens, grew perfect in 25°c (figure 1a). For better data, we grew PF in three different agar plates, PIA, PFA, and TSA (Trypticase Soy Agar). I will be having those results in the coming week.
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| Figure 1a |
Refrence
C D Cox and P Adams (1985) Infection and Immunity 48(1): 130–138
Hey Ibrahim, your project sounds quite exciting! Why exactly are you trying to figure out what kind of plastics ‘Pseudomonas’ grows on? Is this bacteria harmful if humans or animals come into contact with it? I also saw you in the lab trying to look for plastics! What kind of plastics are you planning on using?
ReplyDeleteThe ‘Pseudomonas’ species bacteria is a harmful bacteria when it comes to clinical studies. Common symptoms are nausea, diarrhea, vomiting, etc. So, for your question "Is it harmful to humans?" the symptoms should be a sufficient answer. Our focus became on plastics for one reason only, MICROPLASTICS. Microplastics are everywhere in the world including in our day to day drinking water. That being said, imagine if a bacteria species like ‘Pseudomonas’ grows on plastics, imagine the harm it would cause. As for my plastic, I already found my LPDE 4 plastic. Everything is explained if you go to my blog on week 4.
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